Acetamide Medium

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetamide Medium
Composition per liter:
Agar, noble..................................................................................20.0g
Glucose.......................................................................................20.0g
KH 2 PO 4 ......................................................................................15.0g
CsC1 2  solution..........................................................................12.5mL
Acetamide solution..................................................................10.0mL
CaCl 2 ·2H 2 O solution .................................................................4.1mL
MgSO 4 ·7H 2 O solution...............................................................2.4mL
Trace elements solution .............................................................1.0mL
CsCl 2 Solution:
Composition per 100.0mL:
CsC1 2 ........................................................................................16.84g
Preparation of CsCl 2 Solution: Add CsC1 2  to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Acetamide Solution:
Composition per 100.0mL:
Acetamide...................................................................................5.91g
Preparation of Acetamide Solution: Add acetamide to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C.
CaCl 2 ·2H 2 O Solution:
Composition per 100.0mL:
CaCl 2 ·2H 2 O................................................................................14.7g
Preparation of CaCl 2 ·2H 2 O Solution: Add CaCl 2 ·2H 2 O to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
MgSO 4 ·7H 2 O Solution:
Composition per 100.0mL:
MgSO 4 ·7H 2 O............................................................................24.65g
Preparation of MgSO 4 ·7H 2 O Solution: Add MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution:
Composition per liter:
FeSO 4 ·7H 2 O .................................................................................5.0g
CoCl 2 ·6H 2 O..................................................................................3.7g
MnSO 4 ·1H 2 O................................................................................1.6g
ZnSO 4 ·7H 2 O.................................................................................1.4g
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except CsC1 2  solution,
acetamide solution, CaCl 2 ·2H 2 O solution, and MgSO 4 ·7H 2 O solution,
to distilled/deionized water and bring volume to 971.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 12.5mL of sterile
CsC1 2  solution, 10.0mL of sterile acetamide solution, 4.1mL of sterile
CaCl 2 ·2H 2 O solution, and 2.4mL of sterile MgSO 4 ·7H 2 O solution. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Trichoderma longibra-
chiatum.