Acetamide Nutrient Broth

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetamide Nutrient Broth
Composition per liter:
Acetamide.....................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
NaCl..............................................................................................0.2g
MgSO 4 , anhydrous....................................................................0.158g
Na 2 MoO 4 ·2H 2 O.........................................................................5.0mg
FeSO 4 .........................................................................................0.5mg
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components, except acetamide, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Add acetamide. Adjust pH to 7.0. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use: For the differentiation of nonfermentative Gram-negative bact-
eria, especially Pseudomonas aeruginosa.