FSM Selective Medium

[所属分类:培养基配方] [发布时间:2021-3-4] [发布人: ] [阅读次数:] [返回]

FSM Selective Medium

培养基配方

Composition per liter:
Agar ............................................................................................18.0g
Peptone........................................................................................10.0g
Glucose .........................................................................................4.0g
(NH 4 ) 2 SO 4 ...................................................................................1.32g
K 2 HPO 4 .......................................................................................1.18g
Casamino acids.............................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .......................................................................................0.44g
MgSO 4 ·7H 2 O................................................................................0.2g
FeC 6 H 5 O 7 ·5H 2 O.........................................................................3.0mg
Citric acid...................................................................................1.9mg
ZnSO 4 ·7H 2 O..............................................................................1.6mg
MnSO 4 ·H 2 O...............................................................................1.5mg
2,3,5-Triphenyltetrazolium·HCl solution.................................10.0mL
Benomyl solution.....................................................................10.0mL
Polymyxin B solution ..............................................................10.0mL
Chloroneb solution...................................................................10.0mL
Dichloran solution....................................................................10.0mL
Bacitracin solution...................................................................10.0mL
Cycloheximide solution...........................................................10.0mL
Pentachloronitrobenzene solution............................................10.0mL
Pimaricin solution....................................................................10.0mL
Tyrothricin solution..................................................................10.0mL
Vancomycin solution................................................................10.0mL
Chloromycetin solution............................................................10.0mL
Penicillin G solution ................................................................10.0mL
2,3,5-Triphenyltetrazolium·HCl Solution:
Composition per 10.0mL:
2,3,5-Triphenyltetrazolium·HCl.................................................0.5mg
Preparation of 2,3,5-Triphenyltetrazolium·HCl Solution:
Add 2,3,5-triphenyltetrazolium·HCl to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 7 min at 15 psi pressure–121°C.
Benomyl Solution:
Composition per 10.0mL:
Benomyl.....................................................................................0.5mg
Preparation of Benomyl Solution: Add benomyl to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Polymyxin B Solution:
Composition per 10.0mL:
Polymyxin B..............................................................................0.1mg
Preparation of Polymyxin B Solution: Add polymyxin B to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Chloroneb Solution:
Composition per 10.0mL:
Chloroneb ..................................................................................0.1mg
Preparation of Chloroneb Solution: Add chloroneb to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Dichloran Solution:
Composition per 10.0mL:
Dichloran ...................................................................................0.1mg
Preparation of Dichloran Solution: Add dichloran to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Bacitracin Solution:
Composition per 10.0mL:
Bacitracin.................................................................................0.05mg
Preparation of Bacitracin Solution: Add bacitracin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide.........................................................................0.05mg
Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.
Pentachloronitrobenzene Solution:
Composition per 10.0mL:
Pentachloronitrobenzene..........................................................0.03mg
Preparation of Pentachloronitrobenzene Solution: Add pentachloronitrobenzene to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Pimaricin Solution:
Composition per 10.0mL:
Pimaricin..................................................................................0.02mg
Preparation of Pimaricin Solution: Add pimaricin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Tyrothricin Solution:
Composition per 10.0mL:
Tyrothricin ...............................................................................0.02mg
Preparation of Tyrothricin Solution: Add tyrothricin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Vancomycin Solution:
Composition per 10.0mL:
Vancomycin.............................................................................0.01mg
Preparation of Vancomycin Solution: Add vancomycin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Chloromycetin Solution:
Composition per 10.0mL:
Chloromycetin ............................................................................5.0μg
Preparation of Chloromycetin Solution: Add chloromycetin to distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Penicillin G Solution:
Composition per 10.0mL:
Penicillin G.................................................................................1.0μg
Preparation of Penicillin G Solution: Add penicillin G to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components—except 2,3,5-triphenyltetrazolium chloride solution, benomyl solution, polymyxin B solution, chloroneb solution, dichloran solution, bacitracin solution, cycloheximide solution, pentachloronitrobenzene solution, pimaricin solution, tyrothricin solution, vancomycin solution, chloromycetin solution, and penicillin G solution—to distilled/deionized water and bring volume to 870.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add 10.0mL each of sterile 2,3,5-triphenyltetrazolium chloride solution, benomyl solution, polymyxin B solution, chloroneb solution, dichloran solution, bacitracin solution, cycloheximide solution, pentachloronitrobenzene solution, pimaricin solution, tyrothricin solution, vancomycin solution, chloromycetin solution, and penicillin G solution. Mix thoroughly. Pour into sterile Petri dishes. Dry plates for 24 hr at 30°C.
Use: For the isolation and cultivation of Pseudomonas solanacearum from soil.