FTX Broth

[所属分类:培养基配方] [发布时间:2021-3-4] [发布人: ] [阅读次数:] [返回]

FTX Broth

培养基配方

Composition per 1001.0mL:
Sodium glutamate.......................................................................10.0g
Glucose .........................................................................................2.0g
Tris................................................................................................2.0g
Sodium glycerophosphate.............................................................0.1g
Artificial seawater.........................................................................1.0L
Trace elements solution .............................................................1.0mL
pH 8.0 ± 0.2 at 25°C
Artificial Seawater:
Composition per liter:
NaCl............................................................................................24.7g
MgSO 4 ·7H 2 O................................................................................6.3g
MgCl 2 ·6H 2 O..................................................................................4.6g
CaCl 2 .............................................................................................1.0g
KCl................................................................................................0.7g
NaHCO 3 ........................................................................................0.2g
Preparation of Artificial Seawater: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
Disodium EDTA ...........................................................................8.0g
MnCl 2 ·4H 2 O..................................................................................0.1g
CoCl 2 ·6H 2 O ................................................................................0.02g
KBr..............................................................................................0.02g
KI................................................................................................0.02g
ZnCl 2 ...........................................................................................0.02g
CuSO 4 .........................................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O..........................................................................0.01g
LiCl............................................................................................5.0mg
SnCl 2 ·2H 2 O................................................................................5.0mg
Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to 1.0L of artificial seawater. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation and cultivation of Cytophaga species, Herpetosiphon species, Saprospira species, and Flexithrix species.