Fructose Mineral Medium

[所属分类:培养基配方] [发布时间:2021-3-4] [发布人: ] [阅读次数:] [返回]

Fructose Mineral Medium

培养基配方

Composition per liter:
Solution A..............................................................................500.0mL
Solution B..............................................................................300.0mL
Solution C..............................................................................200.0mL
Solution A:
Composition per 500.0mL:
Na 2 HPO 4 ·12H 2 O.......................................................................... 9.0g
K 2 PO 4 ...........................................................................................1.5g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Trace elements solution SL-6....................................................1.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Solution A:  Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Solution B:
Composition per 300.0mL:
CaCl 2 ·2H 2 O ................................................................................0.01g
Ferric ammonium citrate...........................................................0.005g
Preparation of Solution B:  Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Solution C:
Composition per 200.0mL:
Fructose.........................................................................................5.0g
Preparation of Solution C:  Add fructose to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: To 500.0mL of sterile solution A, aseptically add 300.0mL of sterile solution B and 200.0mL of sterile solution C. Mix thoroughly. Aseptically dispense into sterile tubes or flasks.
Use:  For the general cultivation of a wide variety of bacteria.