Friis Medium (DSMZ Medium 1078)

[所属分类:培养基配方] [发布时间:2021-3-4] [发布人: ] [阅读次数:] [返回]

Friis Medium

(DSMZ Medium 1078)

培养基配方

Composition per 162.0mL:
Beef heart, infusion from..............................................................0.7g
Pancreatic digest of gelatin.........................................................0.32g
Peptone........................................................................................0.27g
Brain heart, solids from infusion ................................................0.13g
NaCl..............................................................................................0.1g
Na 2 HPO 4 .....................................................................................0.08g
Glucose .......................................................................................0.07g
NaCl............................................................................................0.07g
Hank’s balance salt solution (BSS)..........................................50.0mL
Porcine serum, heat inactivated...............................................30.0mL
Yeast extract solution...............................................................3.32mL
Phenol Red solution...............................................................0.133mL
pH 7.4 ± 0.2 at 25°C
Hanks’ Balanced Salt Solution:
Composition per liter:
Solution A................................................................................25.0mL
Solution B................................................................................25.0mL
Solution A:
Composition per 25.0mL :
Na 2 Cl.............................................................................................0.8g
KCl................................................................................................0.4g
CaCl 2 .............................................................................................1.4g
MgCl 2 ·6H 2 O..............................................................................0.185g
MgSO 4 ·7H 2 O................................................................................0.1g
Preparation of Solution A:  Add components to 25.0mL distilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C.
Solution B:
Composition per 25.0mL :
Na 2 HPO 4 ·7H 2 O...........................................................................0.15g
KH 2 PO 4 .......................................................................................0.06g
Preparation of Solution B:  Add components to 25.0mL distilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Hanks’ Balanced Salt Solution: Add solutions A and B to sterile distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Phenol Red Solution:
Composition per 15.0mL :
Phenol Red....................................................................................0.1g
Preparation of Phenol Red Solution:  Add components to
15.0mL distilled/deionized water. Filter sterilize.
Yeast Extract Solution:
Composition per 15.0mL :
Yeast extract..................................................................................2.5g
Preparation of Yeast Extract Solution:  Add components to 15.0mL distilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except porcine serum, yeast extract solution, and Phenol Red solution, to distilled/deionized water and bring volume to 78.0mL. Mix thoroughly. Adjust pH to 7.4. Gently heat while stirring and bring to boiling. Boil for 1 min. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add porcine serum, yeast extract solution, and Phenol Red solution. Bring volume to 162.0mL with distilled/deionized water.
Use: For the cultivation of Mycoplasma spp., e.g., Mycoplasma hyorhinis.