Frankia Medium

[所属分类:培养基配方] [发布时间:2021-3-4] [发布人: ] [阅读次数:] [返回]

培养基配方

Composition per liter:
Basal medium ........................................................................900.0mL
Albumin fatty acid supplement..............................................100.0mL
pH 7.4 ± 0.2 at 25°C
Basal Medium:
Composition per liter:
Na 2 HPO 4 .......................................................................................1.0g
NaCl..............................................................................................1.0g
KH 2 PO 4 .........................................................................................0.3g
Glycerol (10% solution).............................................................1.0mL
NH 4 Cl (25% solution)................................................................1.0mL
Thiamine (0.5% solution)..........................................................1.0mL
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Albumin Fatty Acid Supplement:
Composition per 200.0mL:
Bovine albumin fraction V .........................................................20.0g
Tween™ 80 (10% solution).....................................................25.0mL
FeSO 4 ·7H 2 O (0.5% solution)...................................................20.0mL
CaCl 2 ·2H 2 O (1% solution).........................................................2.0mL
MgCl 2 ·6H 2 O (1% solution)........................................................2.0mL
Vitamin B 12 (0.2% solution)......................................................2.0mL
ZnSO 4 ·7H 2 O (0.4% solution)....................................................2.0mL
CuSO 4 ·5H 2 O (0.3% solution)....................................................0.2mL
Preparation of Albumin Fatty Acid Supplement: Add albumin to 100.0mL of distilled/deionized water. Mix thoroughly. Slowly add Tween™ 80 and then other components. Adjust pH to 7.4. Add distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Aseptically combine 900.0mL of sterile basal medium and 100.0mL of sterile albumin fatty acid supplement. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use:  For the cultivation and maintenance of Frankia species.