Frankia Medium

[所属分类:培养基配方] [发布时间:2021-3-4] [发布人: ] [阅读次数:] [返回]

Frankia Medium

培养基配方

Composition per 1200.0mL:
Na 2 HPO 4 .......................................................................................1.0g
NaCl..............................................................................................1.0g
KH 2 PO 4 .........................................................................................0.3g
Glycerol .....................................................................................1.0mL
NH 4 Cl ........................................................................................1.0mL
Thiamine....................................................................................1.0mL
Albumin fatty acid supplement..............................................200.0mL
pH 7.4 ± 0.2 at 25°C
Albumin Fatty Acid Supplement:
Composition per 200.0mL:
Bovine albumin fraction V .........................................................20.0g
Tween™ 80..............................................................................25.0mL
FeSO 4 ·7H 2 O.............................................................................20.0mL
CaCl 2 ·2H 2 O................................................................................2.0mL
MgCl 2 ·6H 2 O...............................................................................2.0mL
Vitamin B 12 ................................................................................2.0mL
ZnSO 4 ·7H 2 O..............................................................................2.0mL
CuSO 4 ·5H 2 O..............................................................................0.2mL
Preparation of Albumin Fatty Acid Supplement: Add components to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except albumin fatty acid supplement, to distilled/deionized water and bring volume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 200.0mL of sterile albumin fatty acid supplement. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Frankia species.