Fraser Broth

[所属分类:培养基配方] [发布时间:2021-3-4] [发布人: ] [阅读次数:] [返回]

Fraser Broth

培养基配方

Composition per liter:
NaCl............................................................................................20.0g
Na 2 HPO 4 .....................................................................................12.0g
Beef extract...................................................................................5.0g
Proteose peptone...........................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
LiCl...............................................................................................3.0g
KH 2 PO 4 .......................................................................................1.35g
Esculin ..........................................................................................1.0g
Fraser supplement solution......................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Diagnostic Systems.
Fraser Supplement Solution:
Composition per 10.0mL:
Ferric ammonium citrate...............................................................0.5g
Acriflavine·HCl ..........................................................................0.25g
Nalidixic acid................................................................................0.1g
Ethanol.......................................................................................5.0mL
Preparation of Fraser Supplement Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except Fraser supplement solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile Fraser supplement solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the isolation of Listeria species from food and environmental species.