Forget Fredette Agar

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Forget Fredette Agar  

培养基配方 

Composition per liter:
Casein enzymatic hydrolysate ....................................................17.0g
Agar............................................................................................10.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
Glucose.........................................................................................2.5g
K 2 HPO 4 .........................................................................................2.5g
NaN 3 .............................................................................................0.5g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Caution: Sodium azide is toxic. Azides also react with metals and disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the selective isolation of anaerobic microorganisms.