Fomes annosus Isolation Medium No. 2

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Fomes annosus Isolation Medium No. 2 

培养基配方

Composition per liter:
Malt extract.................................................................................20.0g
Agar............................................................................................17.0g
Streptomycin sulfate solution..................................................10.0mL
Phenylphenol solution ...............................................................2.5mL
Lactic acid (50% solution).........................................................1.0mL
Streptomycin Sulfate Solution:
Composition per 10.0mL:
Streptomycin sulfate.....................................................................0.1g
Preparation of Streptomycin Sulfate Solution: Add streptomycin sulfate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Phenylphenol Solution:
Composition per 20.0mL:
o-Phenylphenol...........................................................................0.48g
Ethanol (95% solution)............................................................20.0mL
Preparation of Phenylphenol Solution: Add o-phenylphenol to 20.0mL of ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium: Filter sterilize lactic acid. Add components—except lactic acid, phenylphenol solution, and streptomycin sulfate solution—to distilled/deionized water and bring volume to 986.5mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile lactic acid, phenylphenol solution, and streptomycin sulfate solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Fomes annosus.