Formate Fumarate Medium

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Formate Fumarate Medium 

培养基配方

Composition per 900.0mL:
Agar............................................................................................20.0g
Sodium fumarate.........................................................................16.6g
Sodium formate ............................................................................6.8g
Yeast extract..................................................................................4.0g
Sodium thioglycolate solution...............................................100.0mL
pH 7.3 ± 0.2 at 25°C

Sodium Thioglycolate Solution:
Composition per 100.0mL:
Sodium thioglycolate....................................................................0.5g
Preparation of Sodium Thioglycolate Solution: Add sodium thioglycolate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Sparge with 100% N 2 gas. Warm to 50°–55°C.
Preparation of Medium: Add components, except sodium thioglycolate solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 5 min. Cool to 50°–55°C while sparging with 80% N 2 + 20% CO 2 . Anaerobically distribute 4.5mL volumes into anaerobic tubes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°– 55°C. Prior to inoculation, add 0.5mL of sterile sodium thioglycolate solution to each tube.
Use: For the cultivation and identification of Bacteroides gracilis, Bacteroides ureolyticus, Campylobacter species, and Wolinella species.