Fomes annosus Isolation Medium No. 1

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Fomes annosus Isolation Medium No. 1 

培养基配方

Composition per liter:
Agar ............................................................................................20.0g
Peptone..........................................................................................5.0g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ........................................................................................0.25g
Pentachloronitrobenzene (PCNB)............................................0.19mg
Streptomycin..............................................................................0.1mg
Ethanol (95% solution)............................................................20.0mL
Lactic acid (50% solution).........................................................2.0mL
Preparation of Medium: Filter sterilize ethanol and lactic acid.
Add components, except ethanol and lactic acid, to distilled/deionized water and bring volume to 978.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile ethanol and lactic acid. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Fomes annosus.