Folic AOAC Medium

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Folic AOAC Medium 

培养基配方

Composition per liter:
Sodium citrate.............................................................................52.0g
Glucose .......................................................................................40.0g
Vitamin assay casamino acids.....................................................10.0g
K 2 HPO 4 .........................................................................................6.4g
L- Cysteine·HCl............................................................................0.76g
L- Asparagine .................................................................................0.6g
MgSO 4 ·7H 2 O................................................................................0.4g
L- Tryptophan.................................................................................0.2g
Sorbitan monooleate complex ......................................................0.1g
FeSO 4 ·7H 2 O................................................................................0.02g
MnSO 4 ·7H 2 O..............................................................................0.02g
NaCl............................................................................................0.02g
Xanthine......................................................................................0.02g
Adenine sulfate...........................................................................0.01g
Guanine·HCl...............................................................................0.01g
Uracil ..........................................................................................0.01g
Glutathione.................................................................................5.2mg
Pyridoxine·HCl..........................................................................4.0mg
p-Aminobenzoic acid.................................................................1.0mg
Riboflavin ..................................................................................1.0mg
Calcium pantothenate ................................................................0.8mg
Nicotinic acid.............................................................................0.8mg
Thiamine·HCl ............................................................................0.4mg
Biotin .......................................................................................0.02mg
pH 6.7 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling with frequent mixing. Cool to 45°–50°C. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 5 min at 15 psi pressure– 121°C.
Use: For the microbiological assaying of folic acid using Enterococcus hirae as the test organism.