Endo Agar with Sodium Chloride

[所属分类:培养基配方] [发布时间:2020-7-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Endo Agar with Sodium Chloride

培养基配方
Composition per liter:
Agar............................................................................12.0g
Lactose.........................................................................10.0g
Peptone, special ................................................................8.0g
NaCl.............................................................................3.0g
Na2SO3 ..........................................................................2.5g
K2HPO4 ..........................................................................2.0g
Basic Fuchsin....................................................................0.2g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution: Basic Fuchsin is a potential carcinogen and care must be
taken to avoid inhalation of the powdered dye and contact with the
skin.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Pour into sterile Petri dishes. Swirl flask while pouring plates to
keep precipitate in suspension. Protect from the light.
Use: For the selective isolation, cultivation, and differentiation of coli-
form and other enteric microorganisms based on their ability to ferment
lactose.