Endo Broth (m-Endo Broth)

[所属分类:培养基配方] [发布时间:2020-7-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Endo Broth
(m-Endo Broth)

培养基配方
Composition per liter:
Lactose.......................................................................12.5g
Peptone.......................................................................10.0g
NaCl...........................................................................5.0g
Pancreatic digest of casein....................................................5.0g
Peptic digest of animal tissue.................................................5.0g
K2HPO4 ......................................................................4.375g
Na2SO3 ........................................................................2.1g
Yeast extract..................................................................1.5g
KH2PO4 ......................................................................1.375g
Basic Fuchsin.................................................................1.05g
Sodium deoxycholate............................................................0.1g
Ethanol (95% solution).......................................................20.0mL
pH 7.2 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Basic Fuchsin is a potential carcinogen and care must be
taken to avoid inhalation of the powdered dye and contact with the
skin.
Preparation of Medium: Add ethanol to approximately 900.0mL
of distilled/deionized water. Add remaining components. Bring vol-
ume to 1.0L with distilled/deionized water. Mix thoroughly. Gently
heat and bring to boiling. Rapidly cool broth below 45°C. Do not auto-
clave. Use 1.8–2.0mL for each filter pad. Protect from the light. Pre-
pare broth freshly.
Use: For the cultivation and enumeration of coliform bacteria from
water by the membrane filter method.