Endo Agar, LES (m-Endo Agar, LES)

[所属分类:培养基配方] [发布时间:2020-7-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Endo Agar, LES
(m-Endo Agar, LES)

培养基配方
Composition per liter:
Agar...........................................................................10.0g
Lactose.........................................................................9.4g
Tryptose........................................................................7.5g
NaCl............................................................................3.7g
Peptone ........................................................................3.7g
Pancreatic digest of casein.....................................................3.7g
K2HPO4 .........................................................................3.3g
Na2SO3 .........................................................................1.6g
Yeast extract...................................................................1.2g
KH2PO4 .........................................................................1.0g
Sodium deoxycholate.............................................................0.1g
Sodium lauryl sulfate..........................................................0.05g
Basic Fuchsin solution.........................................................8.0mL
pH 7.2 ± 0.2 at 25°C
Basic Fuchsin Solution:
Composition per 10.0mL:
Basic Fuchsin...................................................................1.0g
Ethanol (95% solution)........................................................10.0mL
Caution: Basic Fuchsin is a potential carcinogen and care must be
taken to avoid inhalation of the powdered dye and contact with the
skin.
Preparation of Basic Fuchsin Solution: Add Basic Fuchsin to
10.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Pour into sterile Petri dishes. Swirl flask while pouring
plates to keep precipitate in suspension. Protect from the light.
Use: For the cultivation and enumeration of coliform bacteria from
water by the membrane filter method.