Endo Agar, LES (Endo Agar, Laurance Experimental Station) (m-Endo Agar, LES) (m-LES, Endo Agar)

[所属分类:培养基配方] [发布时间:2020-7-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Endo Agar, LES
(Endo Agar, Laurance Experimental Station)
(m-Endo Agar, LES)
(m-LES, Endo Agar)

培养基配方
Composition per liter:
Agar ......................................................................14.0g
Lactose.....................................................................9.4g
Peptones (pancreatic digest of casein 65%
and yeast extract 35%)......................................................7.5g
NaCl........................................................................3.7g
Pancreatic digest of casein.................................................3.7g
Peptic digest of animal tissue..............................................3.7g
K2HPO4 .....................................................................3.3g
Na2SO3 .....................................................................1.6g
Yeast extract...............................................................1.2g
KH2PO4 .....................................................................1.0g
Basic Fuchsin...............................................................0.8g
Sodium lauryl sulfate......................................................0.05g
Ethanol...................................................................20.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Basic Fuchsin is a potential carcinogen and care must be
taken to avoid inhalation of the powdered dye and contact with the
skin.
Preparation of Medium:  Add ethanol to approximately 900.0mL
of distilled/deionized water. Add remaining components. Bring vol-
ume to 1.0L with distilled/deionized water. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile 60mm Petri dishes in 4.0mL volumes. Protect
from the light.
Use: For the cultivation and enumeration of coliform bacteria by the
membrane filter method.