A Medium, 5X

[所属分类:培养基配方] [发布时间:2019-9-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

A Medium, 5X
Composition per liter:
K 2 HPO 4 ......................................................................................52.5g
KH 2 PO 4 ......................................................................................22.5g
(NH 4 ) 2 SO 4 ....................................................................................5.0g
Sodium citrate·2H 2 O.....................................................................2.5g
Carbon source solution ............................................................10.0mL
MgSO 4 ·7H 2 O solution...............................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Carbon Source Solution:
Composition per 100.0mL:
Glycerol or sucrose.....................................................................20.0g
Preparation of Carbon Source Solution: Add glycerol or glu-
cose to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
MgSO 4 ·7H 2 O Solution:
Composition per 100.0mL:
MgSO 4 ·7H 2 O............................................................................24.65g
Preparation of MgSO 4 ·7H 2 O Solution: Add MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbon source
solution and MgSO 4 ·7H 2 O solution, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
To prepare medium for use (1×), aseptically dilute 200.0mL of 5× stock
solution with 789.0mL of sterile distilled/deionized water. Aseptically
add 10.0mL of sterile carbon source solution and 1.0mL of sterile
MgSO 4 ·7H 2 O solution. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use: For the cultivation of Escherichia coli.