A 1 Broth

[所属分类:培养基配方] [发布时间:2019-9-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

A 1 Broth
Composition per liter:
Pancreatic digest of casein..........................................................20.0g
Lactose..........................................................................................5.0g
NaCl..............................................................................................5.0g
Salicin ...........................................................................................0.5g
Triton™ X-100 ..........................................................................1.0mL
pH 6.9 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into test tubes containing an inverted Durham
tube. Autoclave for 10 min at 15 psi pressure–121°C.
Use: For the detection of fecal coliforms in foods, treated wastewater,
and seawater by a most-probable-number (MPN) method. Multiple
dilutions of samples (3, 5, or 10 replicates per dilution) are added to
tubes containing A 1 broth. After incubation, test tubes with gas accu-
mulation in the Durham tubes are scored positive and those with no gas
as negative. A MPN table is consulted to determine the most probable
number of fecal coliforms.