EMB HiVeg Agar

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EMB HiVeg Agar

培养基配方
Composition per liter:
Agar ........................................................................13.5g
Plant peptone................................................................10.0g
Lactose......................................................................5.0g
Sucrose......................................................................5.0g
K2HPO4 ......................................................................2.0g
Eosin Y .....................................................................0.4g
Methylene Blue.............................................................0.065g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the differential isolation of Gram-negative enteric bacilli
from clinical and nonclinical specimens. For the isolation, cultivation,
and differentiation of Gram-negative enteric bacteria based on lactose
fermentation. Bacteria that ferment lactose, especially the coliform
bacterium Escherichia coli, appear as colonies with a green metallic
sheen or blue-black to brown color. Bacteria that do not ferment lactose
appear as colorless or transparent, light purple colonies.