​EMB Agar, Modified (Eosin Methylene Blue Agar, Modified)

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EMB Agar, Modified
(Eosin Methylene Blue Agar, Modified)

培养基配方
Composition per liter:
Agar ........................................................................15.0g
Lactose......................................................................10.0g
Pancreatic digest of gelatin.................................................10.0g
K2HPO4 ......................................................................2.0g
Eosin Y .....................................................................0.4g
Methylene Blue.............................................................0.065g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 60°C. Shake medium to oxidize methylene blue. Pour
into sterile Petri dishes. Swirl flask while pouring plates to distribute pre-
cipitate.
Use: For the isolation, cultivation, and differentiation of Gram-nega-
tive enteric bacteria based on lactose fermentation. Bacteria that fer-
ment lactose, especially the coliform bacterium Escherichia coli,
appear as colonies with a green metallic sheen or blue-black to brown
color. Bacteria that do not ferment lactose appear as colorless or trans-
parent, light purple colonies.