EMB HiVeg Agar, Levine

[所属分类:培养基配方] [发布时间:2020-7-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EMB HiVeg Agar, Levine

培养基配方
Composition per liter:
Agar...........................................................................15.0g
Plant peptone .................................................................10.0g
Lactose........................................................................10.0g
K2HPO4 .........................................................................2.0g
Eosin Y.........................................................................0.4g
Methylene Blue................................................................0.065g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the isolation, enumeration, and differentiation of members of
Enterobacteriaceae. For the isolation, cultivation, and differentiation of
Gram-negative enteric bacteria based on lactose fermentation. Bacteria
that ferment lactose, especially the coliform bacterium Escherichia
coli, appear as colonies with a green metallic sheen or blue-black to
brown color. Bacteria that do not ferment lactose appear as colorless or
transparent, light purple colonies.