Egg Yolk Agar, Modified

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Egg Yolk Agar, Modified

培养基配方
Composition per liter:
Agar............................................................................20.0g
Pancreatic digest of casein.....................................................15.0g
Vitamin K 1 ....................................................................10.0g
NaCl.............................................................................5.0g
Papaic digest of soybean meal....................................................5.0g
Yeast extract....................................................................5.0g
L- Cystine.......................................................................0.4g
Hemin...........................................................................5.0mg
Egg yolk emulsion.............................................................100.0mL
Source:  This medium is available as a prepared medium from BD Di-
agnostic Systems.
Egg Yolk Emulsion:
Composition :
Chicken egg yolks...............................................................11
Whole chicken egg...............................................................1
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg.
Preparation of Medium: Add components, except egg yolk emul-
sion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile egg
yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation, cultivation, and differentiation of Clostridium
species and some other anaerobic bacteria.