Egg Yolk Agar Base, HiVeg with Egg Yolk Emulsion

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Egg Yolk Agar Base, HiVeg with Egg Yolk Emulsion

培养基配方
Composition per liter:
Plant peptone No.3........................................................40.0g
Agar......................................................................25.0g
Na2HPO4 ...................................................................5.0g
Glucose....................................................................2.0g
NaCl.......................................................................2.0g
KH2PO4 ....................................................................1.0g
MgSO4 .....................................................................0.1g
Fe4(P2O7)3 ·H2O .........................................................5.0mg
Egg yolk emulsion.......................................................100.0mL
pH 7.6 ± 0.2 at 25°C
Source: This medium, without egg yolk emulsion, is available as a
premixed powder from HiMedia.
Egg Yolk Emulsion:
Composition per liter:
Egg yolks................................................................30.0mL
NaCl, 0.9% solution......................................................70.0mL
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack 11 eggs
and separate yolks from whites. Mix egg yolks. Measure 30.0mL of
egg yolk emulsion and add to 70.0mL of 0.9% sterile NaCl solution.
Mix thoroughly. Warm to 45°–50°C.
Preparation of Medium: Add components, except egg yolk emul-
sion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile egg
yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation, cultivation, and differentiation of Clostridium spe-
cies and some other anaerobic bacteria.