EGGC (DSMZ Medium 1191)

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EGGC
(DSMZ Medium 1191)

培养基配方
Composition per liter:
Agar ........................................................................15.0g
Glucose ......................................................................0.6g
Na-acetate ...................................................................0.3g
Casamino acids ...............................................................0.3g
Salt solution ..............................................................10.0mL
Vitamin solution.............................................................5.0mL
Phosphate soltution..........................................................2.0mL
pH 7.2 ± 0.2 at 25°C
Salt Solution:
Composition per liter:
(NH4)2SO4 ....................................................................1.0g
KCl...........................................................................0.5g
MgSO4 ·7H2O..................................................................0.5g
CaCl2 ·2H2O .................................................................0.2g
CaCO3 ........................................................................0.2g
FeCl3 ·6H2O................................................................0.005g
Preparation of Salt Solution: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per100.0mL:
Thiamine-HCl·2H2O.........................................................50.0mg
Nicotinic acid.............................................................50.0mg
Pyridoxine-HCl.............................................................50.0mg
D -Ca-pantothenate.........................................................50.0mg
Riboflavin ................................................................10.0mg
Vitamin B 12 ...............................................................1.0mg
Folic acid..................................................................0.2mg
Biotin .....................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge
with 80% H2 + 20% CO2 . Filter sterilize.
Phosphate Solution:
Composition per 20.0mL:
Na2HPO4 ....................................................................2.48g
NaH2PO4 ...................................................................0.308g
Preparation of Phosphate Solution:  Add components to dis-
tilled/deionized water and bring volume to 20.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except phosphate and
vitamin solutions, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Adjust pH to 7.2. Gently heat while stirring
and bring to boiling. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add the vita-
min and phosphate solutions. Mix thoroughly. Pour into Petri dishes or
aseptically distribute into sterile tubes.
Use: For the cultivation of Thiothrix spp.