Egg Yolk Agar

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Egg Yolk Agar

培养基配方
Composition per liter:
Proteose peptone No.2.......................................................40.0g
Agar .......................................................................25.0g
Na2HPO4 ....................................................................5.0g
Glucose ....................................................................2.0g
NaCl........................................................................2.0g
KH2PO4 .....................................................................1.0g
MgSO4 ·7H2O................................................................0.1g
Egg yolk emulsion........................................................100.0mL
Hemin solution............................................................1.0mL
pH 7.6 ± 0.2 at 25°C
Hemin Solution:
Composition per 100.0mL:
Hemin.....................................................................0.5g
NaOH (1N solution)......................................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Egg Yolk Emulsion:
Composition :
Chicken egg yolks......................................................11
Whole chicken egg .....................................................1
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg.
Preparation of Medium: Add components, except egg yolk emul-
sion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile egg
yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation, cultivation, and differentiation of Clostridium spe-
cies and some other anaerobic bacteria.