EG Sodium Chloride Medium No. 7 (Ethylene Glycol NaCl Medium No. 7)

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EG Sodium Chloride Medium No. 7
(Ethylene Glycol NaCl Medium No.7)

培养基配方
Composition per liter:
NaCl...........................................................................70.0g
Agar...........................................................................15.0g
K2HPO4 .........................................................................7.5g
KH2PO4 .........................................................................1.0g
(NH4)2SO4 ......................................................................0.8g
MgSO4 ·7H2O....................................................................0.1g
FeSO4 ·7H2O...................................................................0.01g
Ethylene glycol...............................................................10.0mL
Salt solution .................................................................1.0mL
Salt Solution:
Composition per liter:
CaCl2 ·2H2O...................................................................6.0g
ZnSO4 ·7H2O...................................................................4.4g
MnSO4 ·H2O....................................................................3.0g
(NH4)6Mo7O24 ·4H2O...........................................................1.82g
CuCl2 ·2H2O .................................................................0.2g
Preparation of Salt Solution:  Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of ATCC strain 27042.