EG Agar

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EG Agar

培养基配方
Composition per 1055.0mL:
Agar......................................................................15.0g
Proteose peptone No.3.....................................................10.0g
Yeast extract..............................................................5.0g
Na2HPO4 ...................................................................4.0g
Lab Lemco meat extract ....................................................2.4g
Glucose....................................................................1.5g
L -Cysteine·HCl·H2O......................................................0.5g
Soluble starch.............................................................0.5g
L -Cystine.................................................................0.2g
Horse blood..............................................................50.0mL
HCl (1N solution) .......................................................50.0mL
10% Silicone SH 5535......................................................5.0mL
pH 7.6–7.8 at 25°C
Source:  Lab Lemco meat extract is available from Oxoid Unipath.
10% Silicone SH 5535 is available from Toray. Proteose peptone No.
3 is available from BD Diagnostic Systems.
Preparation of Medium:  Add  L -cystine to 50.0mL of 1N HCl. Mix
thoroughly. Add remaining components and bring volume to 950.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C. Aseptically add 50.0mL of horse blood. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Actinomyces naeslundii, Actinomyces visco-
sus, Bacteroides species, Bifidobacterium species, Campylobacter spe-
cies, Clostridium species, Eubacterium species, Fusobacterium necro-
phorum, Fusobacterium nucleatum, Fusobacterium pseudonecro-
phorum, Lactobacillus species, Megasphaera cerevisiae, Megasphaera
elsdenii, Mitsuokella multiacida, Peptostreptococcus species, Prevotella
species, Propionibacterium species, Rikenella microfusus, Selenomonas
species, and Wolinella recta.