Ectothiorhodosynus Medium (DSMZ Medium 1002)

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ectothiorhodosynus Medium
(DSMZ Medium 1002)

培养基配方
Composition per liter:
NaCl......................................................................20.0g
Na-acetate ................................................................1.0g
K2HPO4 ....................................................................0.5g
Na2S2O3 ...................................................................0.5g
MgCl2 ·6H2O...............................................................0.2g
Yeast extract..............................................................0.1g
Vitamin B 12 ............................................................0.02mg
Ammonium chloride solution...............................................10.0mL
Calcium chloride solution................................................10.0mL
Sodium carbonate solution................................................10.0mL
Sodium bicarbonate solution .............................................10.0mL
Na2S·9H2O solution......................................................10.0mL
Trace elements SL-6 solution .............................................1.0mL
pH 9.3 ± 0.2 at 25°C
Trace Elements SL-6 Solution:
Composition per liter:
H3BO3 .....................................................................0.3g
CoCl2 ·6H2O...............................................................0.2g
ZnSO4 ·7H2O...............................................................0.1g
MnCl2 ·4H2O .............................................................0.03g
Na2MoO4 ·H2O.............................................................0.03g
NiCl2 ·6H2O..............................................................0.02g
CuCl2 · 2H2O ............................................................0.01g
Preparation of Trace Elements SL-6 Solution: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Na2S·9H2O Solution :
Composition per 10.0mL:
Na2S·9H2O................................................................0.24g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Sodium Bicarbonate Solution :
Composition per 10.0mL:
NaHCO3 ...................................................................5.0g
Preparation of Sodium Bicarbonate Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Sodium Carbonate Solution:
Composition per 10.0mL:
Na2CO3 ..................................................................5.0g
Preparation of Sodium Carbonate Solution:  Add components
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C.
Calcium Chloride Solution:
Composition per 10.0mL:
CaCl2 ·2H2O ...........................................................0.1g
Preparation of Calcium Chloride Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Ammonium Chloride Solution:
Composition per 10.0mL:
NH4Cl .................................................................0.5g
Preparation of Ammonium Chloride Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except ammonium
chloride, calcium chloride, carbonate, bicarbonate, and sulfide solu-
tions, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Adjust pH to 9.3. Gently heat while stirring and bring to
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add the ammonium chloride, calcium
chloride, carbonate, bicarbonate, and sulfide solutions. Mix thorough-
ly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Ectothiorhodosynus spp.