Edwards Medium HiVeg Base, Modified

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Edwards Medium HiVeg Base, Modified

培养基配方
Composition per liter:
Agar .........................................................................15.0g
Plant extract ................................................................10.0g
Plant peptone.................................................................10.0g
NaCl...........................................................................5.0g
Esculin .......................................................................1.0g
Thallous sulfate..............................................................0.33g
Crystal Violet................................................................1.3mg
Sterile bovine or sheep blood................................................50.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium, without blood, is available as a premixed pow-
der from HiMedia.
Preparation of Medium: Add components, except blood, to dis-
tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 20 min at 10 psi pres-
sure–115°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile bo-
vine blood or sheep blood. Mix thoroughly. Pour into sterile Petri
dishes.
Use: For the selective isolation and cultivation of Streptococcus aga-
lactiae and other streptococci involved in bovine mastitis.