Ectothiorhodospira vacuolata Medium

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ectothiorhodospira vacuolata Medium

培养基配方
Composition per 1002.0mL:
NaCl....................................................................30.0g
NaHCO3 .................................................................3.0g
KH2PO4 .................................................................1.0g
Sodium malate...........................................................1.0g
Na2SO3 ·5H2O ..........................................................0.5g
NH4Cl ..................................................................0.5g
MgCl2 ·6H2O ...........................................................0.2g
CaCl2 ·2H2O ...........................................................0.1g
Na2S·9H2O solution...................................................10.0mL
Trace elements solution SLA...........................................1.0mL
Vitamin solution VA...................................................1.0mL
pH 8.7 ± 0.2 at 25°C
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O............................................................0.5g
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SLA:
Composition per liter:
CuCl2 ·2H2O .......................................................10.0g
FeCl2 ·4H2O........................................................1.8g
H3BO3 ..............................................................0.5g
CoCl2 ·6H2O ......................................................0.25g
ZnCl2 .............................................................0.1g
MnCl2 ·4H2O......................................................70.0mg
Na2MoO4 ·2H2O ...................................................30.0mg
Na2SeO3 ·5H2O....................................................10.0mg
NiCl2 ·6H2O......................................................10.0mg
Preparation of Trace Elements Solution SLA : Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 2.0–3.0.
Vitamin Solution VA:
Composition per 100.0mL:
Nicotinic acid amide..............................................35.0mg
Thiamine dichloride...............................................30.0mg
p-Aminobenzoic acid...............................................20.0mg
Biotin ...........................................................10.0mg
Calcium  DL -pantothenate.........................................10.0mg
Pyridoxal·HCl....................................................10.0mg
Vitamin B 12 ......................................................5.0mg
Preparation of Vitamin Solution VA:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except Na2S·9H2O so-
lution, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 8.7. Filter sterilize. Aseptically add 10.0mL of
sterile Na2S·9H2O solution. Mix thoroughly. Distribute into sterile
tubes or flasks.
Use:  For the cultivation and maintenance of Ectothiorhodospira vac-
uolata.