Ectothiorhodospira Medium, Modified

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ectothiorhodospira Medium, Modified

培养基配方
Composition per liter:
NaCl.......................................................................30.0g
Na2SO4 ....................................................................10.0g
Sodium acetate..............................................................2.0g
KH2PO4 .....................................................................0.8g
Sodium carbonate buffer (1M, pH 9.0).....................................200.0mL
MgCl2 ·6H2O solution.....................................................10.0mL
Na2S·9H2O solution........................................................6.0mL
CaCl2 ·2H2O solution......................................................5.0mL
NH4Cl solution.............................................................4.0mL
SLA trace elements.........................................................1.0mL
VA vitamin solution........................................................1.0mL
pH 9.0 ± 0.2 at 25°C
MgCl2 ·6H2O Solution:
Composition per 10.0mL:
MgCl2 ·6H2O................................................................0.1g
Preparation of MgCl2 ·6H2O Solution: Add MgCl2 ·6H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O..................................................................0.5g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize. Use freshly prepared solution.
CaCl2 ·2H2O Solution:
Composition per 10.0mL:
CaCl2 ·2H2O................................................................0.1g
Preparation of CaCl2 ·2H2O Solution: Add CaCl2 ·2H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
NH4Cl Solution:
Composition per 10.0mL:
NH4Cl .......................................................................2.0g
Preparation of NH4Cl Solution: Add NH4Cl to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
SLA Trace Elements:
Composition per liter:
FeCl2 ·4H2O.................................................................1.8g
H3BO3 .......................................................................0.5g
CoCl2 ·6H2O................................................................0.25g
ZnCl2 .......................................................................0.1g
MnCl2 ·4H2O ...............................................................0.07g
Na2MoO4 ·2H2O..............................................................0.03g
CuCl2 ·2H2O................................................................0.01g
Na2SeO3 ·5H2O..............................................................0.01g
NiCl2 ·6H2O................................................................0.01g
Preparation of SLA Trace Elements: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 2–3.
VA Vitamin Solution:
Composition per 500.0mL:
Nicotinamide..............................................................0.175g
Thiamine·HCl .............................................................0.15g
p-Aminobenzoic acid.........................................................0.1g
Biotin.....................................................................0.05g
Calcium pantothenate ......................................................0.05g
Pyridoxine·2HCl...........................................................0.05g
Cyanocobalamin............................................................0.025g
Preparation of VA Vitamin Solution: Add components to dis-
tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Preparation of Medium: Add components—except MgCl2 ·6H2O
solution, Na2S·9H2O solution, CaCl2 ·2H2O solution, and NH4Cl solu-
tion—to distilled/deionized water and bring volume to 975.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C. Aseptically add 10.0mL of ster-
ile MgCl2 ·6H2O solution, 6.0mL of sterile Na2S·9H2O solution, 5.0mL
of sterile CaCl2 ·2H2O solution, and 4.0mL of sterile NH4Cl solution.
Mix thoroughly. Aseptically distribute into culture bottles. Incubate for
2 days before inoculation.
Use:  For the cultivation and maintenance of Ectothiorhodospira vac-
uolata.