Ectothiorhodospira Medium

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ectothiorhodospira Medium

培养基配方
Composition per liter:
NaCl........................................................................130.0g
Na2SO4 ......................................................................10.0g
Sodium acetate................................................................2.0g
KH2PO4 .......................................................................0.8g
Sodium carbonate buffer, (1M, pH 9.0)......................................200.0mL
MgCl2 ·6H2O solution ......................................................10.0mL
Na2S·9H2O solution..........................................................6.0mL
CaCl2 ·2H2O solution........................................................5.0mL
NH4Cl solution ..............................................................4.0mL
SLA trace elements...........................................................1.0mL
VA vitamin solution..........................................................1.0mL
pH 9.0 ± 0.2 at 25°C
MgCl2 ·6H2O Solution:
Composition per 10.0mL:
MgCl2 ·6H2O .................................................................0.1g
Preparation of MgCl2 ·6H2O Solution: Add MgCl2 ·6H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O...................................................................0.5g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize. Use freshly prepared solution.
CaCl2 ·2H2O Solution:
Composition per 10.0mL:
CaCl2 ·2H2O ................................................................0.1g
Preparation of CaCl2 ·2H2O Solution: Add CaCl2 ·2H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
NH4Cl Solution:
Composition per 10.0mL:
NH4Cl .......................................................................2.0g
Preparation of NH 4 Cl Solution: Add NH4Cl to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
SLA Trace Elements:
Composition per liter:
FeCl2 ·4H2O...............................................................1.8g
H3BO3 .....................................................................0.5g
CoCl2 ·6H2O..............................................................0.25g
ZnCl2 .....................................................................0.1g
MnCl2 ·4H2O .............................................................0.07g
Na2MoO4 ·2H2O............................................................0.03g
CuCl2 ·2H2O..............................................................0.01g
Na2SeO3 ·5H2O............................................................0.01g
NiCl2 ·6H2O..............................................................0.01g
Preparation of SLA Trace Elements: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 2–3.
VA Vitamin Solution:
Composition per 500.0mL:
Nicotinamide............................................................0.175g
Thiamine·HCl ...........................................................0.15g
p-Aminobenzoic acid.......................................................0.1g
Biotin ..................................................................0.05g
Calcium pantothenate ....................................................0.05g
Pyridoxine·2HCl.........................................................0.05g
Cyanocobalamin .........................................................0.025g
Preparation of VA Vitamin Solution: Add components to dis-
tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Preparation of Medium: Add components—except MgCl2 ·6H2O
solution, Na2S·9H2O solution, CaCl2 ·2H2O solution, and NH 4 Cl solu-
tion—to distilled/deionized water and bring volume to 975.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C. Aseptically add 10.0mL of ster-
ile MgCl2 ·6H2O solution, 6.0mL of sterile Na2S·9H2O solution, 5.0mL
of sterile CaCl2 ·2H2O solution, and 4.0mL of sterile NH4Cl solution.
Mix thoroughly. Aseptically distribute into culture bottles. Incubate for
2 days before inoculation.
Use:  For the cultivation and maintenance of Ectothiorhodospira
abdelmalekii and Ectothiorhodospira halochloris.