Ectothiorhodospira Medium

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ectothiorhodospira Medium

培养基配方
Composition per liter:
NaCl......................................................................180.0g
Na2SO4 ....................................................................20.0g
NaHCO3 ....................................................................14.0g
Na2CO3 .....................................................................6.0g
Sodium succinate............................................................1.0g
NH4Cl ......................................................................0.8g
KH2PO4 .....................................................................0.5g
MgCl2 ·6H2O................................................................0.1g
CaCl2 ·2H2O...............................................................0.05g
Feeding solution..........................................................10.0mL
Trace elements solution SLA................................................1.0mL
Vitamin solution VA .......................................................1.0mL
pH 8.5–8.7 at 25°C
Feeding Solution:
Composition per 100.0mL:
NaCl......................................................................10.0g
NaHCO3 ...................................................................10.0g
Na2S·9H2O.................................................................5.0g
Preparation of Feeding Solution:  Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Trace Elements Solution SLA:
Composition per liter:
FeCl2 ·4H2O..............................................................1.8g
H3BO3 ....................................................................0.5g
CoCl2 ·6H2O ............................................................0.25g
ZnCl2 ...................................................................0.1g
MnCl2 ·4H2O.............................................................0.07g
NaMoO4 ·2H2O............................................................0.03g
CuCl2 ·2H2O ............................................................0.01g
Na2 SeO3 ................................................................0.01g
NiCl2 ·6H2O.............................................................0.01g
Preparation of Trace Elements Solution SLA:  Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 3.0 with 2N HCl.
Vitamin Solution VA:
Composition per liter:
Nicotinamide...........................................................0.04g
Thiamine dichloride....................................................0.03g
p-Aminobenzoic acid....................................................0.02g
Biotin ................................................................0.01g
Calcium pantothenate ..................................................0.01g
Pyridoxal chloride.....................................................0.01g
Vitamin B 12 ..........................................................5.0mg
Preparation of Vitamin Solution VA: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium:  Add components, except trace elements
solution SLA and feeding solution, to distilled/deionized water and
bring volume to 999.0mL. Mix thoroughly. Filter sterilize. Aseptically
add 1.0mL of sterile trace elements solution SLA. Mix thoroughly.
Aseptically distribute into flasks or bottles. Completely fill bottles with
medium except for a pea-sized air bubble. Prior to inoculation, asepti-
cally remove a sufficient amount of medium to permit the addition of
feeding medium. Add 1.0mL of feeding solution per each 100.0mL of
medium.
Use: For the isolation and cultivation of Ectothiorhodospira halochloris
and Ectothiorhodospira halophila.