Ectothiorhodospira halophila Medium

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ectothiorhodospira halophila Medium

培养基配方
Composition per liter:
NaCl........................................................................220.0g
Potassium succinate...........................................................1.0g
Na2S·9H2O....................................................................0.1g
K2HPO4 solution.............................................................20.0mL
NaHCO3 solution.............................................................20.0mL
Solution C..................................................................20.0mL
(NH4)2SO4 solution..........................................................5.0mL
Vitamin solution............................................................0.5mL
pH 7.4–8.0 at 25°C
K2HPO4 Solution:
Composition per liter:
K2HPO4 ....................................................................125.0g
Preparation of K2HPO4 Solution: Add K2 HPO4 to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
NaHCO3 Solution:
Composition per liter:
NaHCO3 ....................................................................100.0g
Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Solution C:
Composition per liter:
MgCl2 ·6H2O .................................................................24.0g
CaCl2 ·2H2O .................................................................3.3g
FeCl3 ·4H2O..................................................................1.1g
(NH4)6 Mo7O24 ·4H2O..........................................................0.1g
Nitrilotriacetic acid......................................................10.0mg
Trace elements solution....................................................50.0mL
Preparation of Solution C: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per 100.0mL:
ZnCl2 ........................................................................0.52g
EDTA..........................................................................0.25g
MnCl2 ·4H2O .................................................................0.08g
FeCl3 ·4H2O..................................................................0.03g
Co(NO3)2 ·6H2O...............................................................0.02g
CuCl2 ·2H2O..................................................................0.02g
H3BO3 ........................................................................0.01g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.0 with 2N HCl.
(NH4)2SO4 Solution:
Composition per liter:
(NH4)2SO4 .................................................................100.0g
Preparation of (NH4)2SO4 Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Nicotinic acid.............................................................2.0mg
Thiamine...................................................................1.0mg
p-Aminobenzoic acid........................................................0.2mg
Biotin....................................................................0.02mg
Vitamin B 12 .............................................................1.0μg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4–8.0.
Filter sterilize. Aseptically distribute into flasks or bottles. Completely
fill bottles with medium except for a pea-sized air bubble.
Use: For the isolation and cultivation of Ectothiorhodospira halo-
phila.