Ectothiorhodospira halophila Medium

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ectothiorhodospira halophila Medium

培养基配方
Composition per liter:
NaCl.........................................................................200.0g
NH4Cl ........................................................................0.4g
(NH4)2SO4 ....................................................................0.1g
Na2CO3 solution ...........................................................100.0mL
Tris buffer (1M solution, pH 7.5)...........................................30.0mL
Solution C..................................................................5.0mL
Potassium phosphate buffer (1M solution, pH 7.5)............................3.0mL
Additional solution.........................................................2.5mL
pH 7.4–8.0 at 25°C
Na2CO3 Solution:
Composition per 100.0mL:
Na2CO3 ....................................................................10.0g
Preparation of Na2CO3 Solution:  Add Na2CO3 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Solution C:
Composition per liter:
MgCl2 ·6H2O...............................................................24.0g
CaCl2 ·2H2O................................................................3.3g
FeCl3 ·4H2O...............................................................1.1g
(NH4)6Mo7O24 ·4H2O........................................................0.1g
Nitrilotriacetic acid...................................................10.0mg
Trace elements solution ................................................50.0mL
Preparation of Solution C: Add nitrilotriacetic acid to 500.0mL of
distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH.
Add remaining components. Readjust pH to 7.2 with H 2 SO 4 or KOH.
Add distilled/deionized water to 1.0L.
Trace Elements Solution:
Composition per 100.0mL:
ZnCl2 .......................................................................0.52g
EDTA.........................................................................0.25g
MnCl2 ·4H2O.................................................................0.08g
FeCl3 ·4H2O.................................................................0.03g
Co(NO3)2 ·6H2O .............................................................0.02g
CuCl2 ·2H2O ................................................................0.02g
H3BO3 .......................................................................0.01g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.0 with 2N HCl.
Additional Solution:
Composition per 50.0mL:
NaS2O3 ·6H2O................................................................6.0g
Sodium succinate.............................................................5.0g
Sodium ascorbate.............................................................1.0g
Preparation of Additional Solution: Add components to dis-
tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except Na2CO3 solu-
tion and additional solution, to distilled/deionized water and bring vol-
ume to 900.0mL. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically adjust pH to 7.4–7.8 with filter-sterilized
HCl. Aseptically distribute into 50.0mL screw-capped bottles. Fill
each bottle almost to the top, leaving a space of 2.8mL in the neck.
Aseptically add 2.5mL of sterile additional solution to each bottle. Mix
thoroughly.
Use: For the isolation and cultivation of Ectothiorhodospira halo-
phila.