Echinamoeba Agar (ATCC Medium 2339)

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Echinamoeba Agar
(ATCC Medium 2339)

培养基配方
Composition per liter:
Agar ..........................................................................18.0g
FeCl2 ·6H2O..................................................................0.552g
MgSO4 ·7H2O....................................................................0.5g
CaSO4 ·2H2O...................................................................0.49g
NaHCO3 ........................................................................0.37g
CaCl2 ·2H2O...................................................................0.24g
Yeast extract..................................................................0.2g
KCl............................................................................8.6mg
KNO3 .........................................................................0.16mg
Mineral solution..............................................................10.0mL
Glycerol ......................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Mineral Solution:
Composition per liter:
ZnSO4 ·7H2O....................................................................3.5g
Na2MoO4 ·2H2O .................................................................0.4g
MnCl2 ·4H2O...................................................................0.25g
(NH4)2Ni(SO4)2 ·6H2O ..........................................................0.2g
LiCl............................................................................0.3g
SnCl2 ·2H2O....................................................................0.1
Preparation of Mineral Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 with
NaOH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C.
Use:  For the cultivation and maintenance of Echinamoeba spp.