ECD MUG Agar

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ECD MUG Agar

培养基配方
Composition per liter:
Casein peptone..............................................................20.0g
Agar........................................................................15.0g
NaCl.........................................................................5.0g
Lactose......................................................................5.0g
K2HPO4 ......................................................................4.0g
Bile salt mixture............................................................1.5g
KH2PO4 ......................................................................1.5g
Tryptophan...................................................................1.0g
4-Methylumbelliferyl-β- D -glucuronide.....................................0.07g
pH 7.0 ± 0.2 at 37°C
Source: This medium is available from Fluka, Sigma-Aldrich.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Pour into sterile Petri dishes.
Use: For detection of Escherichia coli in a variety of specimens. The
bile-salt mixture in this E. coli Direct Agar extensively inhibits the
non-obligatory intestinal accompanying flora. Fluorescence in the UV
and a positive indole test demonstrate the presence of E. coli in the col-
onies.