Echinamoeba Agar

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Echinamoeba Agar

培养基配方
Composition per liter:
Agar ......................................................................18.0g
FeCl2 ·6H2O..............................................................0.552g
MgSO4 ·7H2O...............................................................0.5g
CaSO4 ·2H2O..............................................................0.49g
NaHCO3 ...................................................................0.37g
CaCl2 ·2H2O..............................................................0.24g
Yeast extract.............................................................0.2g
KCl......................................................................8.6mg
KNO3 ...................................................................0.16mg
Mineral solution........................................................10.0mL
Glycerol ................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Mineral Solution:
Composition per liter:
ZnSO4 ·7H2O..............................................................3.5g
Na2MoO4 ·2H2O ...........................................................0.4g
MnCl2 ·4H2O ............................................................0.25g
(NH4)2 Ni(SO4)2 ·6H2O....................................................0.2g
LiCl......................................................................0.3g
SnCl2 ·2H2O..............................................................0.1g
Preparation of Mineral Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 with
NaOH. Gently heat and bring to boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri
dishes or leave in tubes.
Use:  For the cultivation and maintenance of Echinamoeba spp.