​ECD Agar, Fluorocult ® (Fluorocult ECD Agar)

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ECD Agar, Fluorocult ®
(Fluorocult ECD Agar)

培养基配方
Composition per liter:
Peptone from casein ..........................................................20.0g
Agar..........................................................................15.0g
NaCl...........................................................................5.0g
Lactose .......................................................................5.0g
K2HPO4 ........................................................................4.0g
Bile salt mixture..............................................................1.5g
KH2PO4 ........................................................................1.5g
Tryptophan.....................................................................1.0g
4-Methylumbelliferyl-ß-D-glucuronide..........................................0.07g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from Merck.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri
dishes. The prepared broth is clear and yellowish brown.
Use:  For the detection of E. coli in meats. The medium complies with
the German-DIN-Norm 10110 for the examination of meat, with the
regulations according to § 35 LMBG (06.00/36) for the examination of
food, and with ISO Standard 6391 (1996) for the enumeration of E. coli
in meat and meat products. The bile salt mixture of this medium largely
inhibits the accompanying flora not usually found in the intestines.
Using fluorescence under UV light and a positive indole reaction, E.
coli colonies can be identified among the grown colonies.