​Eagle Medium, Modified

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Eagle Medium, Modified

培养基配方
Composition per liter:
Eagle MEM (10X)..........................................................100.0mL
Fetal bovine serum.......................................................100.0mL
Glucose solution .........................................................20.0mL
HEPES (N-2-hydroxyethyl
piperazine-N′-2-ethanesulfonic acid)
buffer, 1M, pH 7.2........................................................20.0mL
Glutamine solution........................................................10.0mL
NaHCO3 (7.5% solution).....................................................7.5mL
Gentamicin sulfate solution................................................0.2mL
pH 7.2 ± 0.2 at 25°C
Eagle MEM (10X):
Composition per 100.0mL:
Sterile salt solution ..................................................97.0mL
TC amino acids, minimal Eagle 50X........................................2.0mL
TC vitamins, minimal Eagle 100X..........................................1.0mL
Preparation of Eagle MEM (10X): Combine components. Mix
thoroughly. Filter sterilize.
Sterile Salt Solution:
Composition per 100.0mL:
NaCl......................................................................6.8g
Glucose...................................................................1.0g
KCl.......................................................................0.4g
CaCl2 ....................................................................0.2g
MgCl2 ....................................................................0.2g
NaH2PO4 ..................................................................0.15g
Preparation of Sterile Salt Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
TC Amino Acids (50X):
Composition per liter:
L- Arginine...............................................................0.1g
L- Lysine................................................................0.06g
L- Isoleucine............................................................0.05g
L- Leucine ..............................................................0.05g
L- Threonine.............................................................0.05g
L- Valine................................................................0.05g
L- Tyrosine .............................................................0.04g
L- Phenylalanine.........................................................0.03g
L- Histidine.............................................................0.03g
L- Cystine...............................................................0.02g
L- Methionine............................................................0.02g
L- Tryptophan............................................................0.01g
Preparation of TC Amino Acids (50X): Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 7.2–7.4. Filter sterilize.
TC Vitamins, Minimal Eagle 100X:
Composition per liter:
Inositol...................................................................2.0mg
Calcium pantothenate ......................................................1.0mg
Choline chloride...........................................................1.0mg
Folic acid ................................................................1.0mg
Nicotinamide...............................................................1.0mg
Pyridoxal..................................................................1.0mg
Thiamine·HCl .............................................................1.0mg
Riboflavin ................................................................0.1mg
Preparation of TC Vitamins, Minimal Eagle 100X : Add com-
ponents to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Filter sterilize.
Glucose Solution:
Composition per 100.0mL:
Glucose .................................................................27.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Glutamine Solution:
Composition per 10.0mL:
L- Glutamine..............................................................5.0g
Preparation of Glutamine Solution: Add glutamine to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Gentamicin Solution:
Composition per 1.0mL:
Gentamicin sulfate.......................................................0.05g
Preparation of Gentamicin Solution: Add gentamicin sulfate to
distilled/deionized water and bring volume to 1.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Combine components. Mix thoroughly.
Filter sterilize.
Use: For the cultivation of animal tissue culture cell lines, especially
for McCoy cells.