Eagle’s Minimal Essential Medium with Earle’s Salts and Nonessential Amino Acids (MEM with Earle’s Salts and Nonessential Amino Acids) (BAM M46)

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Eagle’s Minimal Essential Medium
with Earle’s Salts and Nonessential Amino Acids
(MEM  with Earle’s Salts and
Nonessential Amino Acids)
(BAM M46)

培养基配方
Composition per liter:
NaCl..........................................................................6.8g
NaHCO3 .......................................................................2.2g
Glucose ......................................................................1.0g
KCl...........................................................................0.4g
CaCl2 ·2H2O................................................................0.265g
MgSO4 ·7H2O..................................................................0.2g
L- Arginine·H2O.............................................................0.15g
NaH2PO4 ·H2O................................................................0.14g
L- Arginine·HCl............................................................0.126g
L- Lysine·HCl..............................................................72.5mg
L- Tyrosine, disodium salt..................................................52.1mg
L- Leucine..................................................................52.0mg
L- Threonine................................................................48.0mg
L- Valine ..................................................................46.0mg
L- Histidine·HCl·H2O......................................................42.0mg
D- Phenylalanine............................................................32.0mg
L- Cysteine·2HCl..........................................................31.29mg
L- Methionine...............................................................15.0mg
L- Glutamic acid............................................................14.7mg
L- Aspartic acid ...........................................................13.3mg
L- Proline..................................................................11.5mg
L- Serine ..................................................................10.5mg
L- Tryptophan...............................................................10.0mg
L- Alanine...................................................................8.9mg
Phenol Red..................................................................10.0mg
L- Glycine...................................................................7.5mg
i-Inositol...................................................................2.0mg
D- Calcium pantothenate......................................................1.0mg
Choline chloride.............................................................1.0mg
Folic acid ..................................................................1.0mg
Nicotinamide.................................................................1.0mg
Pyridoxal·HCl...............................................................1.0mg
Thiamine·HCl................................................................1.0mg
Riboflavin...................................................................0.1mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium:  Add components to 1.0L of distilled/de-
ionized water. Mix thoroughly. Filter sterilize.
Use : For the cultivation of animal cells in tissue culture, for example,
cells for viral detection and identification by characteristic cytopathic
effects.