Eagle Medium

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Eagle Medium

培养基配方
Composition per 100.1mL:
Eagle MEM in Earle BSS.............................................94.0mL

NaHCO3 (7.5% solution)..............................................3.0mL
Fetal bovine serum, inactivated.....................................2.0mL
Penicillin-streptomycin solution....................................1.0mL
Amphotericin B solution.............................................0.1mL
pH 7.2–7.4 at 25°C
Eagle MEM in Earle BSS:
Composition per liter:
NaCl.................................................................6.8g
Glucose .............................................................1.0g
KCl..................................................................0.4g
CaCl2 ·2H2O.........................................................0.2g
MgCl2 ·6H2O.........................................................0.2g
NaH2PO4 ............................................................0.15g
L- Arginine .........................................................0.1g
L- Lysine...........................................................0.06g
L- Isoleucine.......................................................0.05g
L- Leucine..........................................................0.05g
L- Threonine........................................................0.05g
L- Valine ..........................................................0.05g
L- Tyrosine.........................................................0.04g
L- Phenylalanine....................................................0.03g
L- Histidine........................................................0.03g
L- Cystine .........................................................0.02g
L- Methionine.......................................................0.02g
L- Tryptophan.......................................................0.01g
i-Inositol..........................................................2.0mg
Calcium pantothenate ...............................................1.0mg
Choline chloride....................................................1.0mg
Folic acid..........................................................1.0mg
Nicotinamide........................................................1.0mg
Pyridoxal...........................................................1.0mg
Thiamine·HCl ......................................................1.0mg
Riboflavin .........................................................0.1mg
Preparation of Eagle MEM in Earle BSS: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Penicillin-Streptomycin Solution:
Composition per 1.0mL:
Streptomycin........................................................0.01g
Penicillin........................................................10,000U
Preparation of Penicillin-Streptomycin Solution: Add compo-
nents to distilled/deionized water and bring volume to 1.0mL. Mix
thoroughly.
Amphotericin B Solution:
Composition per 1.0mL:
Amphotericin B.....................................................1.0mg
Preparation of Amphotericin B Solution: Add amphotericin B
to distilled/deionized water and bring volume to 1.0mL. Mix thoroughly.
Preparation of Medium: Combine components. Mix thoroughly.
Filter sterilize.
Use: For the cultivation of animal tissue culture cell lines.