E Agar (m-E Agar)

[所属分类:培养基配方] [发布时间:2020-7-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

E Agar
(m-E Agar)

培养基配方
Composition per liter:
Yeast extract.............................................................30.0g
Agar .....................................................................15.0g
NaCl......................................................................15.0g
Pancreatic digest of gelatin..............................................10.0g
Esculin ...................................................................1.0g
Nalidixic acid............................................................0.25g
NaN3 .....................................................................0.15g
Cycloheximide.............................................................0.05g
TTC solution.............................................................15.0mL
pH 7.1 ± 0.2 at 25°C
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
TTC Solution:
Composition per 15.0mL:
2,3,5-Triphenyltetrazolium chloride......................................0.15g
Preparation of TTC Solution: Add triphenyltetrazolium chloride
to distilled/deionized water and bring volume to 15.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except TTC solution, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add sterile TTC solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Use: For the isolation, cultivation, and enumeration of enterococci in
water by the membrane filter method. It is used in conjunction with
esculin iron agar.