E. coli O157:H7 MUG Agar

[所属分类:培养基配方] [发布时间:2020-7-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

E. coli O157:H7 MUG Agar

培养基配方
Composition per liter:
Casein peptone..............................................................20.0g
Agar........................................................................13.0g
Sorbitol ...................................................................10.0g
NaCl.........................................................................5.0g
Meat extract.................................................................2.0g
Na2S2O3 .....................................................................2.0g
Na-deoxycholate.............................................................1.12g
Yeast extract................................................................1.0g
Ammonium ferric citrate .....................................................0.5g
4-Methylumbelliferyl-β- D -glucuronide......................................0.1g
Bromthymol Blue............................................................0.025g
pH 7.4 ± 0.2 at 37°C
Source: This medium is available from Fluka, Sigma-Aldrich.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Pour into sterile Petri dishes.
Use: For the isolation and differentiation of enterohemorrhagic
(EHEC) E. coli O157:H7 strains from food and clinical specimens.