DYAA LUP

[所属分类:培养基配方] [发布时间:2020-7-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

DYAA LUP

培养基配方
Composition per liter:
Agar ....................................................................20.0g
Glucose .................................................................10.0g
Yeast extract.............................................................1.0g
Asparagine................................................................0.5g
K2HPO4 ·3H2O.............................................................0.5g
MgSO4 ·7H2O.............................................................0.25g
FeCl3  solution..........................................................0.5mL
Lupine stems..........................................................variable
FeCl3 Solution:
Composition per 10.0mL:
FeCl3 ...................................................................1.0g
Preparation of FeCl3 Solution: Add FeCl3  to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add components, except lupine stems, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute 6.0mL volumes into tubes.
Cut lupine stems into 8.0cm-long pieces. Add 2–3 lupine stems per
tube. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to
cool in a slanted position.
Use:  For the cultivation of Ceratocystis coerulescens, Ceratocystis
microspora, Coniella pulchella, Phoma lini, and Phoma glomerata.