Dunkelberg Carbohydrate Medium, Modified

[所属分类:培养基配方] [发布时间:2020-7-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dunkelberg Carbohydrate Medium, Modified

培养基配方
Composition per 100.0mL:
Proteose peptone No.3.....................................................1.5g
Carbohydrate..............................................................1.0g
Na2HPO4 ·2H2O..........................................................0.207g
Phenol Red..............................................................0.055g
NaH2PO4 ·H2O...........................................................0.038g
Horse serum..............................................................5.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 95.0mL. For carbohydrate,
use glucose, maltose, or starch. Mix thoroughly. Filter sterilize. Asepti-
cally add sterile horse serum. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use:  For the cultivation and differentiation of Gardnerella vaginalis
based on its ability to ferment glucose, maltose, or starch.