Dung Extract Agar (DSMZ Medium 781)

[所属分类:培养基配方] [发布时间:2020-7-1] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dung Extract Agar
(DSMZ Medium 781)

培养基配方
Composition per liter:
Agar ..........................................................................15.0g
Malt extract....................................................................5.0g
(NO3)2 ·4H2O..................................................................0.72g
MgSO4 ·7H2O....................................................................0.5g
K2HPO4 ........................................................................0.25g
Peptone ........................................................................0.1g
Dung extract.................................................................100.0mL
pH 6.9 ± 0.2 at 25°C
Dung Extract:
Composition per 150.0mL:
Horse dung.................................................................variable
Preparation of Dung Extract:  Add an average sized piece of
horse dung to150.0mL water. Gently heat and bring to boiling. Boil for
2 hr in a water bath. Filter. Use immediately.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Panaeolus cyanescens.