Dermocystidium Medium

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dermocystidium Medium

培养基配方
Composition per liter:
NaCl...................................................................48.0g
Agar ..................................................................36.0g
MgSO4 ·7H2O...........................................................16.0g
Glucose ................................................................8.0g
Casein hydrolysate or sodium glutamate..................................4.0g
Tris(hydroxymethyl)aminomethane buffer..................................4.0g
KCl.....................................................................1.4g
CaCl2 .................................................................0.94g
K2HPO4 ................................................................0.86g
Thiamine·HCl ......................................................400.0μg
Cyanocobalamine.......................................................6.0μg
Trace metal mix stock.................................................20.0mL
Trace Metal Mix Stock:
Composition per 100.0mL:
H3BO3 ................................................................114.0mg
EDTA..................................................................100.0mg
FeCl3 ·6H2O...........................................................96.8mg
MnCl2 ·4H2O...........................................................36.0mg
Na2MoO4 ·2H2O ........................................................23.0mg
ZnCl2 .................................................................13.4mg
CuCl2 ·2H2O .........................................................536.0μg
CoCl2 ·6H2O .........................................................400.0μg
pH 7.4 ± 0.3 at 25°C
Preparation of Trace Metal Mix Stock:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Adjust pH to 7.4 with concentrated HCl. Bring volume to 2.0L
with distilled/deionized water. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave
in tubes.
Use: For the cultivation and maintenance of Dermocystidium species.